University of Alexandria
Medical Research Institute
Medical Technology Center
Immunology unit
Hands on: BASICS
Techniques
In
Cell Culture
And
STEM CELL Research
Organized By
Prof. Mohamed Samy Afifi M.D, Ph.D
(Boston University, USA)
Medical Research Institute
Alexandria University
22-24 April 2014
3-Day Cell Culture
The purpose of the Cell Culture Workshop is to provide a solid understanding of basic cell culture techniques through classroom and laboratory work. Attendants will gain significant experience in the manipulation of cells through culture. Through integrated learning techniques, utilizing hands-on training, and lectures, attendants will learn the biological basis and relevance of the process of culturing cells as well as the basis of stem cell research. This will allow interested attendants to translate the techniques and information learned in this workshop into their own laboratories
This workshop provides prerequisite training for the culture of numerous cell types.
This workshop provides prerequisite training for the culture of numerous cell types.
Who should attend?
Personnel in the fields of Immunology, Virology, Microbiology, as well as all laboratory personnel dealing with in-vitro fertilizations, and tissue typing. Also, pharmaceutical laboratories personnel and members of the Egyptian Society of Immunology.
Course Programme
09:30 AM – 11:30 AM Lectures
11:30 AM – 12:00 PM Break
12:00 PM – 04:00 PM Practical work
09:30 AM – 11:30 AM Lectures
11:30 AM – 12:00 PM Break
12:00 PM – 04:00 PM Practical work
A certificate will be awarded upon completion of the course
Topics Covered
1) Introduction to cell and tissue culture.
2) Serum-containing and Serum-free culture media.
3) Trypsinization.
4) Cell counting, viability and growth curves.
5) Growth of adherent and non- adherent cells.
6) Suspension culture differentiation and attachment.
7) Adaptation to suspension culture.
8) Adaptation to suspension serum-free media.
9) Cryo preservation and cryo thawing.
10) Cell cloning, primary culture and the cell cycle.
11) Stem Cells (types & origins).
12) Mesenchymal stem cells.
13) Cord blood transplantation.
1) Introduction to cell and tissue culture.
2) Serum-containing and Serum-free culture media.
3) Trypsinization.
4) Cell counting, viability and growth curves.
5) Growth of adherent and non- adherent cells.
6) Suspension culture differentiation and attachment.
7) Adaptation to suspension culture.
8) Adaptation to suspension serum-free media.
9) Cryo preservation and cryo thawing.
10) Cell cloning, primary culture and the cell cycle.
11) Stem Cells (types & origins).
12) Mesenchymal stem cells.
13) Cord blood transplantation.
Speakers
Teaching Staff of the Medical Research Institute, Alexandria University.
Registration Fees
850 LE for Egyptian university staff members
1200 LE for others
700 $ for non-Egyptian
850 LE for Egyptian university staff members
1200 LE for others
700 $ for non-Egyptian
Accommodation
is available upon prior request of at least one month before the course start date:
100 LE for Single room/ day
is available upon prior request of at least one month before the course start date:
100 LE for Single room/ day
175 LE for Double room/ day
For further enquiries, please contact
Mrs. Mai Kotb Tel: 01063436912
Add: 71 Vector Emanuel St., Smouha, Alexandria
Phone : 03-4266213/17/18 FAX : 03-4266214
E-mail : medrinal@hotmail.com
Mrs. Mai Kotb Tel: 01063436912
Add: 71 Vector Emanuel St., Smouha, Alexandria
Phone : 03-4266213/17/18 FAX : 03-4266214
E-mail : medrinal@hotmail.com
